Cell line (origin) protocol passage before transplant site of transplant transplanted cells mouse strain: sex:
age: condition		 histology after -3 months histology after 6- months pathological diagnosis
Human fetal frontal lobe (9 weeks old) oh-NSC-7-fb p13 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#3
#4
#5
#6
pathological
diagnosis
Human fetal frontal lobe (9 weeks old) oh-NSC-7-fb p13 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b4
#r1
#r2
#r4
#r5
#r6
pathological
diagnosis
Human fetal hindbrain (5-7 weeks old) SAI-1 p27 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#3
pathological
diagnosis
Human fetal hindbrain (5-7 weeks old) SAI-1 p28 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#r1
#r3
#r4
pathological
diagnosis
Human adult PBMC-derivediPSC (1201C1) NR-NSPC p12 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#3
#5
#6
#4
pathological
diagnosis
Human adult PBMC-derivediPSC (1201C1) NR-NSPC p12 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b10
#b2
#b4
#b7
#r2
#r6
pathological
diagnosis
Human adult PBMC-derivediPSC (1231A3) EB-NSPC p7 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#3
#4
#5
#6
pathological
diagnosis
Human adult PBMC-derivediPSC (1231A3) EB-NSPC p7 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b2
#b4
#r1
#b6
#r3
pathological
diagnosis
Human adult dermal fibroblast-dericed iPSC lt-NESC AF22 p37 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#4
#5
#6
pathological
diagnosis
Human adult PBMC-derivediPSC (1201C1) EB-NSPC p8 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b6
#r10
#r6
pathological
diagnosis
Human adult dermal fibroblast-dericed iPSC lt-NESC AF22 p37 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b6
#b7
#b8
pathological
diagnosis
Human adult PBMC-derivediPSC (1201C1) EB-NSPC p8 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#3
#4
#5
#6
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) lt-NESC (EGF-) p10 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b2
#b7
#r3
#r4
#r7
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) lt-NESC (EGF-) p8 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#3
#4
#5
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) lt-NESC (EGF-) p20 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#3
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) lt-NESC (EGF-) p20 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b7
#r2
#r5
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) lt-NESC (EGF+) p7,8 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b8
#b9
#r1
#r2
#r5
#r9
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) lt-NESC (EGF+) p11 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#2
#4
#6
#1
#3
#5
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) lt-NESC (EGF+) p20 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#3
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) lt-NESC (EGF+) p20 Spinal cord, epicenter of injury 1x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b1
#b10
#b5
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) EB-NSPC p8 Striatum, bilateral 1x10^6/mouse NOG mouse : female :
9 weeks old : intact
#2
#3
#5
#1
#4
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) EB-NSPC p8 Spinal cord, epicenter of injury 5x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#1
#3
#7
#5
#9
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) NR-NSPC p10 Striatum, bilateral 2x10^6/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#4
#1
#3
#5
pathological
diagnosis
Human adult PBMC-derivediPSC (1210B2) NR-NSPC p10 Spinal cord, epicenter of injury 5x10^5/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#6
#8
#1
#10
pathological
diagnosis
Human adult PBMC-derivediPSC (1231A3) NR-NSPC p10 Striatum, bilateral 1x10^5/mouse NOG mouse : female :
9 weeks old : intact
#1
#2
#3
#4
#5
#6
pathological
diagnosis
Human adult PBMC-derivediPSC (1231A3) NR-NSPC p10 Spinal cord, epicenter of injury 1.4x10^6/mouse NOD/SCID mouse : female :
9 weeks old : SCI9pod
#b3
#b5
#b6
#r4
#r5
#r7
pathological
diagnosis
Mouse fetal NSPC (ffLuc+) Transgenic mice cp156-derived, GFP+ p3 Striatum, bilateral 2x10^6/mouse B6J : female :
9 weeks old : 9days after SCI
#2
#3
#4
#5
pathological
diagnosis
Human adult dermal fibroblast-dericed iPSC lt-NESC AF22 p54 Striatum, bilateral 2x10^6/mouse NOG : female :
10 weeks old : intact
#1
#3
#2
#5
pathological
diagnosis
Human adult dermal fibroblast-dericed iPSC lt-NESC AF22 p55 Striatum, bilateral 2x10^6/mouse NOG : female :
9 weeks old : intact
#2
#4
pathological
diagnosis
Human adult dermal fibroblast-dericed iPSC (409B2) lt-NESC p24 Striatum, bilateral 2x10^6/mouse NOG : female :
9 weeks old : intact
#1
#2
#3
#4
#5
pathological
diagnosis

*NSPC: neural stem/progenitor cells
*SCI: spinal cord injury (contusion injury, 60-70kdn by IH impacter)
*PBMC: peropheral blood derived mononuclear cell
*oh-NSC-7-fb: Kanemura et al, Journal of Neuroscience Research, 2002
*SAI-1: Tailor et al, Journal of Neuroscience, 2013
*AF22: Falk et al, Plos ONE, 2012

Sugai K, Fukuzawa R, Shofuda T, Fukusumi H, Kawabata S, Nishiyama Y, Kawai K, Isoda M, Kanematsu D, Hashimoto-Tamaoki T, Kohyama J, Iwanami A, Suemizu H, Ikeda E, Matsumoto M, Kanemura Y, Nakamura M, Hideyuki O. Pathological classification of human iPSC-derived neural stem/progenitor cells towards safety assessment of transplantation therapy for CNS diseases. Molecular Brain. 2016 Sep 19;9(1):85. doi: 10.1186/s13041-016-0265-8. [PubMed]


Isoda M, Kohyama J, Iwanami A, Sanosaka T, Sugai K, Yamaguchi R, Matsumoto T, Nakamura M, Okano H. Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions. Neurosci Res. 2016 Sep;110:18-28. doi: 10.1016/j.neures.2016.04.003. Epub 2016 Apr 13. [PubMed]

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またコンテンツは、営利目的を除き、転載・リンクすることができます。使用する場合は、使用先のテキスト、図版、画像、映像などに当ウェブサイトのURL、もしくは該当情報の原著論文(Sugai et al., Molecular Brain, 2016)を記載していただくようお願いいたします。

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お問い合わせ

ご意見・お問い合わせフォームよりご連絡ください。

助成元クレジット

This work was supported in part by grants from the Japan Science and
Technology (JST)-California Institute for Regenerative Medicine (CIRM)
Collaborative Program; Grants-in-Aid for Scientific Research from the Japan
Society for the Promotion of Science (SPS) and the Ministry of Education,
Culture, Sports, Science and Technology of Japan (MEXT); and a grant from
the Research Center Network for Realization of Regenerative Medicine from
JST and Japan Agency for Medical Research and Development (AMED) (to H.O.,
M.N. and Y.K.).  H.O. is a paid scientific consultant for San Bio, Co.,
Ltd. All other authors declare that they have no competing financial interests.
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