SKIP005850 - SKIP Search

SKIP005850 : MUSli009-A-1

Cell Line
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Cell Line Name	MUSli009-A-1
Original Database URL
Database
Cell Line Name(2)	p.A735V-SCN5A hiPSC_4.3
Species	Human
Type	iPS Cell
Original Tissue	Skin dermal fibroblasts
Disease Status	Normal
Description	Human induced pluripotent stem cells (hiPSCs) derived from dermal fibroblasts having wild type (WT) SCN5A were engineered by CRISPR/Cas9-mediated genome editing to harbor a specific point mutation (C2204>T) in SCN5A, which results in a substitution of the WT alanine by valine at codon 735 (A735V). The established MUSli009-A-1 hiPSC line has a homozygous C2204>T mutation on exon 14 of SCN5A that was confirmed by DNA sequencing analysis. The cells exhibited normal karyotype, expressed pluripotent markers and retained its capability to differentiate into three germ layers. The cardiomyocytes derived from this line would be a useful model for investigating cardiac channelopathy.
Cell Morphology	human ES-like
Grade	Research Grade

Method for Establishment

Vector	Retrovirus
Feeder Cell	No
Medium	Nutristem XF
Genome Editing	point mutation C2204>T in SCN5A gene
CO2(%)	5%

Detection of Contaminants

Mycoplasma	Negative	Method

Pluripotency Tests

Pluripotent Markers	Yes	Assay
in vitro Differentiation	Yes	Assay
in vivo Differentiation	Yes	Assay

Chromosomal QC

Karyotype	Yes	Assay

Cell Identity

STR	Yes	Type	**********

No data available

Age	--
Sex	Male
Race	Unknown

No data available

Contact Information

#	Name	Organization	Contact Email
1	SudjitLuanpitpong	Siriraj Center of Excellence for Stem Cell Research (SiSCR), Faculty of Medicine Siriraj Hospital, Mahidol University

Information Provider

#	Name	Organization	Contact Email
1	SudjitLuanpitpong	Siriraj Center of Excellence for Stem Cell Research (SiSCR), Faculty of Medicine Siriraj Hospital, Mahidol University

Provider/Distributor

#	Availability	Organization	Email	URL
1	Available	Siriraj Center of Excellence for Stem Cell Research (SiSCR), Faculty of Medicine Siriraj Hospital, Mahidol University