SKIP005465 : iPS-MEF-Fb/Ng-440A-3
| Cell Line Name | iPS-MEF-Fb/Ng-440A-3 |
|---|---|
| Original Database URL | |
| Database | |
| Cell Line Name(2) | APS0003 |
| Species | Mouse |
| Type | iPS Cell |
| Original Tissue | Skin embryonic fibroblast |
| Disease Status | Normal |
| Description | |
| Cell Morphology | mouse ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | Plasmid | |
|---|---|---|
| Transgene | Oct3/4, Sox2, Klf4, c-Myc, Fbx15βgeo/βgeo, Nanog-GFP-IRES-Puror | |
| Feeder Cell | Yes | SNL 76/7 (X-rays:5000R or MMC) 1-2x10^(6) cells/100mm dish |
| Medium | DMEM(high glucose, without Sodium Pyruvate)+15%FBS+0.1mM NEAA+0.1mM 2-Mercaptoethanol+1000U/ml mouse LIF | |
| CO2(%) | 5% | |
Detection of Contaminants
| Mycoplasma | Negative | Method | Staining(Vero),PCR |
|---|
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | RT-PCR |
|---|---|---|---|
| in vitro Differentiation | No | Assay | |
| in vivo Differentiation | Yes | Assay | Teratoma assay |
Chromosomal QC
| Remaining Vector Detection Test | No | Type |
|---|
No data available
| Age | -- | ||
|---|---|---|---|
| Sex | Female | ||
| Race | Unknown | ||
Journal
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Yamanaka, Shinya | CiRA , Kyoto University |
Information Provider
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Yamanaka, Shinya | CiRA , Kyoto University |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Available | CiRA , Kyoto University | http://www.cira.kyoto-u.ac.jp/ | |
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