SKIP003168 : VSMUi001-D
| Cell Line Name | VSMUi001-D |
|---|---|
| Original Database URL | |
| Database | |
| Cell Line Name(2) | PR39 |
| Species | Pig |
| Type | iPS Cell |
| Original Tissue | Skin Fibroblast |
| Disease Status | Normal |
| Description | |
| Cell Morphology | mouse ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | Retrovirus | |
|---|---|---|
| Transgene | OCT4, SOX2, KLF4, c-MYC and LIN28 | |
| Feeder Cell | Yes | mouse embryonic fibroblasts (MEFs) |
| Medium | Knock-out DMEM (KO-DMEM, Invitrogen) supplemented with 20% FBS (HyClone), 0.1 mM non-essential amino acids (NEAA, Invitrogen), 1 mM L-glutamine, 10 ng LIF (Millipore, ESG1106), 10 ng bFGF (Millipore, GF003), 0.1 mM β-mercaptoethanol and 50 units/50 mg/ml penicillin/streptomycin | |
| Genome Editing | No | |
| CO2(%) | 5% | |
Detection of Contaminants
| Mycoplasma | Negative | Method | PCR |
|---|
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | Immunofluorescence, RT-PCR |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | EB formation, spontaneous cardiac differentiation |
| in vivo Differentiation | Yes | Assay | teratoma assay |
Chromosomal QC
| Karyotype | Yes | Assay | G-banding |
|---|
No data available
| Age | -- | ||
|---|---|---|---|
| Sex | Male | ||
| Race | Unknown | ||
Journal
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Sasitorn Rungarunlert | Faculty of Veterinary Science, Mahidol University |
Information Provider
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Sasitorn Rungarunlert | Faculty of Veterinary Science, Mahidol University |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Available | Faculty of Veterinary Science, Mahidol University |