SKIP003167 : ESi062-A
| Cell Line Name | ESi062-A |
|---|---|
| Original Database URL | |
| Database | |
| Cell Line Name(2) | Ctrl2-FiPS5F2 |
| Species | Human |
| Type | iPS Cell |
| Original Tissue | Skin Foreskin fibroblasts |
| Disease Status | Normal |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | mRNA | |
|---|---|---|
| Transgene | OCT4, SOX2, KLF4, LIN28, c-MYC | |
| Feeder Cell | Yes | Human foreskin fibroblasts (HFF) |
| Medium | KO DMEM, KSR 20%, Glutamax 2 mM, non-essential amino acids 0.1 mM, β-mercaptoethanol 0.23 mM, basic FGF 10 ng/mL, penicillin/streptomycin | |
| Genome Editing | No | |
| CO2(%) | 5% | |
Detection of Contaminants
| Mycoplasma | Negative | Method | MycoAlertTM(Lonza) |
|---|
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | Immunofluorescence, RT-PCR, flow cytometry |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | embryoid body formation |
| in vivo Differentiation | Yes | Assay | teratoma assay |
Chromosomal QC
| Karyotype | Yes | Assay | G-banding |
|---|
Cell Identity
| STR | Yes | Type | ********** |
|---|
No data available
| Age | 4 | ||
|---|---|---|---|
| Sex | Male | ||
| Race | Unknown | ||
Journal
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Dunja Lukovic | Research Center Principe Felipe |
Information Provider
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Dunja Lukovic | Research Center Principe Felipe |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Available | Research Center Principe Felipe |