SKIP003165 : NERCe002-A-1
| Cell Line Name | NERCe002-A-1 |
|---|---|
| Original Database URL | |
| Database | |
| Cell Line Name(2) | chHES-90-PLEX-ZsGreen |
| Species | Human |
| Type | ES Cell |
| Original Tissue | In vitro fertilization-derived embryo |
| Disease Status | Normal |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | Lentivirus | |
|---|---|---|
| Transgene | Zoanthus sp. green fluorescent protein (ZsGreen) | |
| Feeder Cell | Yes | mouse embryonic fibroblasts (MEFs) |
| Medium | knock-out Dulbecco's modified Eagle's medium (DMEM)/F12 supplemented with 15% knockout serum replacement, 0.1 mM β-mercaptoethanol, 2 mM non-essential amino acids, 2 mM l-glutamine, and 4 ng/mL human recombinant basic fibroblast growth factor (bFGF) (all from Invitrogen, Carlsbad, CA, USA) | |
| CO2(%) | 5% | |
Detection of Contaminants
| Mycoplasma | Negative | Method | MycoAlertTM(Lonza) |
|---|
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | Immunofluorescence, RT-PCR |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | directed differentiation |
| in vivo Differentiation | Yes | Assay | teratoma assay |
Chromosomal QC
| Karyotype | Yes | Assay | G-banding |
|---|
Cell Identity
| STR | Yes | Type | ********** |
|---|
No data available
| Age | -- | ||
|---|---|---|---|
| Sex | Male | ||
| Race | Asian : Chinese | ||
Journal
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Yi Sun | National Engineering and Research Center of Human Stem Cells |
Information Provider
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Yi Sun | National Engineering and Research Center of Human Stem Cells |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Available | National Engineering and Research Center of Human Stem Cells |