SKIP003091 - SKIP Search

SKIP003091 : ESi054-A

Cell Line
Donor
Clinical Observation
Quality Control
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Cell Line Name	ESi054-A
Original Database URL
Database
Cell Line Name(2)	AS FiPS1 Ep6F 2
Species	Human
Type	iPS Cell
Original Tissue	Skin Fibroblast
Disease Status	Diseased
Description	iPS cell line derived from an autosomal recessive Alport syndrome (ARAS) patient with the homozygous COL4A3 mutation c.345delG, p.(P166Lfs*37).
Cell Morphology	human ES-like
Grade	Research Grade

Method for Establishment

Vector	Plasmid
Transgene	OCT3/4, SOX2, KLF4, LIN28, L-MYC, p53 shRNA
Feeder Cell	No
Medium	mTeSR1 medium
Genome Editing	No
CO2(%)	5%

Detection of Contaminants

Mycoplasma	Negative	Method	PCR

Pluripotency Tests

Pluripotent Markers	Yes	Assay	Immunofluorescence
in vitro Differentiation	Yes	Assay	embryoid body formation

Chromosomal QC

Karyotype	Yes	Assay	G-banding
Remaining Vector Detection Test	Yes	Type	qRT-PCR

Cell Identity

STR	Yes	Type	**********

No data available

Disease Name	autosomal recessive Alport syndrome (ARAS)	ICD10	Q87.8
Disease Name	autosomal recessive Alport syndrome (ARAS)	OMIM ID	203780
Age	25
Sex	Female
Race	Caucasian : Chinese
Genetic Analysis	Yes

No data available

Journal

1	Stem Cell Res. 2017;25:1-5.

Contact Information

#	Name	Organization	Contact Email
1	Anna Veiga	Center of Regenerative Medicine in Barcelona

Information Provider

#	Name	Organization	Contact Email
1	Angel Raya	Center of Regenerative Medicine in Barcelona (CMRB)

Provider/Distributor

#	Availability	Organization	Email	URL
1	Available	Center of Regenerative Medicine in Barcelona