SKIP003036 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19)

Cell Line NameDnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19)
Original Database URL
Database
Cell Line Name(2)AES0146
SpeciesMouse
TypeES Cell
Original Tissueblastocyst
Disease StatusUnknown
Description
Cell Morphologymouse ES-like
GradeResearch Grade

Method for Establishment

VectorPlasmid
TransgeneLacZ, Neomycin resistance gene, Hygromycin resistance gene, Puromycin resistance gene, IRES
Feeder Cell--
MediumGMEM+15%FBS+0.1mM NEAA+1mM Sodium Pyruvate+1000U/ml mouse LIF+0.1mM 2-Mercaptoethanol
Genome EditingLacZ, Neomycin resistance gene, Hygromycin resistance gene, Puromycin resistance gene, IRES
CO2(%)5%

Detection of Contaminants

Mycoplasma Negative Method Staining(Vero)
No data available
Age--
SexMale
Race129Sv/Jae
No data available

Contact Information

# Name Organization Contact Email
1 Okano, Masaki Laboratory for Mammalian Epigenetic Studies, Center for Developmental Biology, RIKEN

Provider/Distributor

# Availability Organization Email URL
1 Available RIKEN BRC http://www2.brc.riken.jp/lab/cell/detail.cgi?cell_no=AES0146&type=1