SKIP003015 - SKIP Search

SKIP003015 : FUS P525L-GFP

Cell Line
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Cell Line Name	FUS P525L-GFP
Original Database URL
Database
Species	Human
Type	iPS Cell
Original Tissue	Skin fibroblast
Disease Status	Diseased→Genome editing
Description	Isogenic iPSC line generated from an iPSC clone derived from a ALS patient carrying a R521C mutation. The patient-specific FUS mutation was corrected at its mutation site and simultaneously a new (P525L) mutation carrying an additional c-terminal GFP tag was added.
Cell Morphology	human ES-like
Grade	Research Grade

Method for Establishment

Vector	Retrovirus
Transgene	OCT4, SOX2, KLF4 and cMYC
Feeder Cell	Yes	MMC-treated CF-1 feeder cells (Globalstem)
Medium	ES-Media containing 5 ng/ml FGF2
Genome Editing	Yes (CRISPR/Cas9)

Detection of Contaminants

Mycoplasma	Negative	Method	PCR

Pluripotency Tests

Pluripotent Markers	Yes	Assay	Immunofluorescence
in vitro Differentiation	Yes	Assay	embryoid body formation

Chromosomal QC

Karyotype	Yes	Assay	HumanCytoSNP-12v array

No data available

Disease Name	Amyotrophic lateral sclerosis (ALS)	ICD10	G12.2
Disease Name	Amyotrophic lateral sclerosis (ALS)	OMIM ID	608030
Age	65
Sex	Female
Race	Unknown
Genetic Analysis	Yes

No data available

Journal

1	Nat Commun. 2018;9(1):335.

Contact Information

#	Name	Organization	Contact Email
1	Andreas Hermann	Department of Neurology, Technische Universitat Dresden

Information Provider

#	Name	Organization	Contact Email
1	Andreas Hermann	Department of Neurology, Technische Universitat Dresden

Provider/Distributor

#	Availability	Organization	Email	URL
1	Information Only