SKIP002977 : CSSi002-A (2851)

Cell Line NameCSSi002-A (2851)
Original Database URL
Database
SpeciesHuman
TypeiPS Cell
Original TissueSkin fibroblast
Disease StatusDiseased
Description
Cell Morphologyhuman ES-like
GradeResearch Grade

Method for Establishment

VectorPlasmid
TransgeneL-MYC, LIN28, SOX2, KLF4, and OCT4 combined with a short hairpin RNA for P53 (shP53)
Feeder CellNo
MediumNutristemXF medium (Biological Industries)
Genome EditingNo
CO2(%)5%

Detection of Contaminants

Mycoplasma Negative Method EZ-PCR

Pluripotency Tests

Pluripotent MarkersYes Assay Immunofluorescence, qPCR
in vitro DifferentiationYes Assay embryoid body formation
in vivo DifferentiationYes Assay teratoma assay

Chromosomal QC

KaryotypeYes Assay G-banding
Remaining Vector Detection TestYes Type qPCR

Cell Identity

STRYes Type **********

No data available
Disease NameHuntington DiseaseICD10G10
OMIM ID143100
Age8
SexFemale
RaceCaucasian
Genetic Analysis Yes
No data available

Contact Information

# Name Organization Contact Email
1 Jessica Rosati Cellular Reprogramming Unit, IRCCS Casa Sollievo della Sofferenza

Information Provider

# Name Organization Contact Email
1 Angelo Luigi Vescovi Cellular Reprogramming Unit, IRCCS Casa Sollievo della Sofferenza

Provider/Distributor

# Availability Organization Email URL
1 Available Cellular Reprogramming Unit, IRCCS Casa Sollievo della Sofferenza