SKIP002922 : Dnmt3b-/- ES (clone 8bb)
| Cell Line Name | Dnmt3b-/- ES (clone 8bb) |
|---|---|
| Original Database URL | |
| Database | |
| Cell Line Name(2) | AES0165 |
| Species | Mouse |
| Type | ES Cell |
| Original Tissue | blastocyst |
| Disease Status | Unknown |
| Description | |
| Cell Morphology | mouse ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | -- | |
|---|---|---|
| Transgene | IRES-βgeo, PGK-hygromycin | |
| Feeder Cell | Yes | MEF (X-rays:5000R or MMC) 3-5x10^(5) cells/60mm dish. |
| Medium | GMEM+10%FBS+0.1mM NEAA+1mM Sodium Pyruvate+1000U/ml mouse LIF+0.1mM 2-Mercaptoethanol | |
| CO2(%) | 5% | |
Detection of Contaminants
| Mycoplasma | Negative | Method |
|---|
No data available
| Age | -- | ||
|---|---|---|---|
| Sex | Male | ||
| Race | strain | ||
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | En Li & Okano, Masaki | RIKEN BioResource Center |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Available | RIKEN BioResource Center | http://ja.brc.riken.jp/ |