SKIP001755 - SKIP Search

SKIP001755 : iPS-L1

Cell Line
Donor
Clinical Observation
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Cell Line Name	iPS-L1
Original Database URL
Database
Cell Line Name(2)	APS0008
Species	Rabbit
Type	iPS Cell
Original Tissue	Liver
Disease Status	Normal
Description	Rabbit induced pluripotent stem (iPS) cell line.
Cell Morphology	human ES-like
Grade	Research Grade

Method for Establishment

Vector	Lentivirus
Transgene	pCSII-EF-MCS-IRES2-Venus lentiviral vector, human-Oct3/4, -Sox2, -Klf4, -c-Myc
Feeder Cell	Yes	MEF
Medium	DMEM/HamF12(2mM L-Glutamine)+20%KSR+0.1mM NEAA+0.1mM 2-Mercaptoethanol+8ng/ml human bFGF
CO2(%)	6%

Detection of Contaminants

Mycoplasma	Negative	Method	Staining(Vero),PCR

Pluripotency Tests

Pluripotent Markers	Yes	Assay	alkaline phosphatase (AP) activity,RT-PCR,immunostaining
in vitro Differentiation	Yes	Assay	EB fomation
in vivo Differentiation	Yes	Assay	Teratoma fomation

Chromosomal QC

Karyotype	Yes	Assay	Unknown

No data available

Age	--
Sex	Unknown
Race	Unknown

No data available

Journal

1	J Biol Chem. 2010;285(41):31362-9.

Contact Information

#	Name	Organization	Contact Email
1	Honda, Arata	RIKEN BioResource Center

Provider/Distributor

#	Availability	Organization	Email	URL
1	Available	RIKEN BioResource Center		http://ja.brc.riken.jp/
	Terms of Use
	Terms of Use	1) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the approver . 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref.(J Biol Chem. 2010 ; 285 : 31362-9) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 5) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.