SKIP001419 : CMESC 40
| Cell Line Name | CMESC 40 |
|---|---|
| Original Database URL | |
| Database | |
| Cell Line Name(2) | No.40 |
| Species | non-human primate Common Marmoset |
| Type | ES Cell |
| Original Tissue | Blastocyst |
| Disease Status | Normal |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | -- | |
|---|---|---|
| Feeder Cell | Yes | gamma-ray-irradiated MEF |
| Medium | 80% Knockout DMEM supplemented with 20% Knockout Serum Replacement (KSR; Invitrogen), 1 mM L-glutamine, 0.1 mM MEM nonessential amino acids, 0.1 mM 2-mercaptoethanol (2-ME; Sigma), 100 IU/ml penicillin, 100 ug/ml streptomycin sulfate, 250 ng/ml amphotericin B, and 10 ng/ml leukemia inhibitory factor | |
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | Immunostaining |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | EB formation, RT-PCR |
| in vivo Differentiation | Yes | Assay | Teratoma formation |
| Other(1) | Yes | Assay | Telomerase activity assay |
| Other(2) | Yes | Assay | SDIA method for neuronal differentiation |
| Other(3) | Yes | Assay | Hematopoietic differentiation |
Chromosomal QC
| Karyotype | Yes | Assay |
|---|
No data available
| Age | -- | ||
|---|---|---|---|
| Sex | Female | ||
| Race | Unknown | ||
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Hideyuki Okano | Keio university |
Information Provider
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Hideyuki Okano | Keio university |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Available | Keio university | http://www.okano-lab.com/ |