SKIP001151 : SEES6
| Cell Line Name | SEES6 |
|---|---|
| Original Database URL | |
| Database | |
| Species | Human |
| Type | ES Cell |
| Original Tissue | frozen human embryo |
| Disease Status | Normal |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | -- | |
|---|---|---|
| Feeder Cell | Yes | inactivated human mesenchymal stem cell (hMSC) |
| Medium | 15% Knockout SR XenoFree CTS (KO-SR XF; Life Technologies; #12618-013), 85% KO-DMED, 2 mM GlutaMAX-I, 0.1 mM NEAAs, penicillin/streptomycin, 50 μg/mL l-ascorbic acid 2-phosphate (Sigma-Aldrich; #A4544), 10 ng/mL heregulin-1β, recombinant human NRG-beta 1/HRG-beta 1 EGF domain (R&D Systems, MN, USA; #396-HB-050/CF), 200 ng/mL LONG R3-IGF1, recombinant human insulin-like growth factor-1 (Sigma-Aldrich; #85580C), and recombinant human full-length bFGF (Life Technologies; #PHG0261) at 20 ng/mL. | |
| CO2(%) | 5% | |
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | FACS, immunostaining |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | EB formation |
| in vivo Differentiation | Yes | Assay | teratoma formation |
| Other(1) | Yes | Assay | DMB-HPLC |
| Other(2) | Yes | Assay | ABO typing |
Chromosomal QC
| Karyotype | Yes | Assay | G-band |
|---|
Cell Identity
| STR | Yes | Type | ********** |
|---|---|---|---|
| HLA | Yes | Type | ********** |
No data available
| Age | -- | ||
|---|---|---|---|
| Sex | Male | ||
| Race | Unknown | ||
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Umezawa Akihiro | National Research Institute for Child Health and Development |
Information Provider
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Umezawa Akihiro | National Research Institute for Child Health and Development |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Information Only |