SKIP001148 : SEES3

Cell Line NameSEES3
Original Database URL
Database
Cell Line Name(2)HES0008
SpeciesHuman
TypeES Cell
Original Tissuefrozen human embryo
Disease StatusNormal
Description
Cell Morphologyhuman ES-like
GradeClinical Grade

Method for Establishment

Vector--
Feeder CellYesMEF (X-rays:5000R or MMC) 4-5x10^(5) cells/60mm dish
MediumiPSellon+10ng/ml human bFGF
CO2(%)5%

Pluripotency Tests

Pluripotent MarkersYes Assay FACS, immunostaining
in vitro DifferentiationYes Assay EB formation
in vivo DifferentiationYes Assay teratoma formation
Other(1)Yes Assay DMB-HPLC
Other(2)Yes Assay ABO typing

Chromosomal QC

KaryotypeYes Assay G-band

Cell Identity

STRYes Type **********
HLAYes Type **********

No data available
Age--
SexMale
RaceUnknown
No data available

Contact Information

# Name Organization Contact Email
1 Umezawa Akihiro National Research Institute for Child Health and Development

Information Provider

# Name Organization Contact Email
1 Umezawa Akihiro National Research Institute for Child Health and Development

Provider/Distributor

# Availability Organization Email URL
1 Available RIKEN BRC http://www2.brc.riken.jp/lab/cell/detail.cgi?cell_no=HES0008&type=1