SKIP001147 : SEES2
| Cell Line Name | SEES2 |
|---|---|
| Original Database URL | |
| Database | |
| Cell Line Name(2) | HES0007 |
| Species | Human |
| Type | ES Cell |
| Original Tissue | frozen human embryo |
| Disease Status | Normal |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Clinical Grade |
Method for Establishment
| Vector | -- | |
|---|---|---|
| Feeder Cell | Yes | MEF (X-rays:5000R or MMC) 4-5x10^(5) cells/60mm dish |
| Medium | iPSellon+10ng/ml human bFGF | |
| CO2(%) | 5% | |
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | FACS, immunostaining |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | EB formation |
| in vivo Differentiation | Yes | Assay | teratoma formation |
| Other(1) | Yes | Assay | DMB-HPLC |
| Other(2) | Yes | Assay | ABO typing |
Chromosomal QC
| Karyotype | Yes | Assay | G-band |
|---|
Cell Identity
| STR | Yes | Type | ********** |
|---|---|---|---|
| HLA | Yes | Type | ********** |
No data available
| Age | -- | ||
|---|---|---|---|
| Sex | Female | ||
| Race | Unknown | ||
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Umezawa Akihiro | National Research Institute for Child Health and Development |
Information Provider
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Umezawa Akihiro | National Research Institute for Child Health and Development |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Available | RIKEN BRC | http://www2.brc.riken.jp/lab/cell/detail.cgi?cell_no=HES0007&type=1 |