SKIP000989 : BJ3
| Cell Line Name | BJ3 |
|---|---|
| Original Database URL | |
| Database | |
| Species | Human |
| Type | iPS Cell |
| Original Tissue | Skin Fibroblast |
| Disease Status | Normal |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | Retrovirus | |
|---|---|---|
| Transgene | OCT4, SOX2, KLF4, and MYC | |
| Feeder Cell | Yes | mEFs |
| Medium | DMEM/F12, 20% knockout serum replacement, 1 mM L-glutamine, 0.1 mM non-essential amino acids, beta-ME and 10 ng/ml FGF2 | |
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | Immunocytochmeistry |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | differentiation assay to neural cells |
| Other(1) | No | Assay | |
| Other(2) | No | Assay | |
| Other(3) | No | Assay |
Chromosomal QC
| Karyotype | Yes | Assay | |
|---|---|---|---|
| Remaining Vector Detection Test | Yes | Type | RT-PCR |
Stem Cell Transcriptome analysis
| Stem Cell Transcriptome analysis | Yes | Assay | qRT-PCR |
|---|
No data available
| Age | 0 | ||
|---|---|---|---|
| Sex | Male | ||
| Race | Unknown | ||
Journal
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Tony Hunter | Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States |
Information Provider
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Tony Hunter | Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Information Only | Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States |