SKIP000984 : BJ1

Cell Line NameBJ1
Original Database URL
Database
SpeciesHuman
TypeiPS Cell
Original TissueSkin fibroblast
Disease StatusNormal
Description
Cell Morphologyhuman ES-like
GradeResearch Grade

Method for Establishment

VectorRetrovirus
TransgeneOCT4, SOX2, KLF4, and MYC
Feeder CellYesmEFs
MediumDMEM/F12, 20% knockout serum replacement, 1 mM L-glutamine, 0.1 mM non-essential amino acids, beta-ME and 10 ng/ml FGF2

Pluripotency Tests

Pluripotent MarkersYes Assay Immunohistochemistry
Other(1)Yes Assay Differentiation
Other(2)Yes Assay RT-PCR
Other(3)No Assay

Chromosomal QC

KaryotypeYes Assay
Remaining Vector Detection TestYes Type RT-PCR

No data available
Age0-9
SexMale
RaceUnknown
No data available

Journal

Contact Information

# Name Organization Contact Email
1 Tony Hunter Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States

Information Provider

# Name Organization Contact Email
1 Tony Hunter Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States

Provider/Distributor

# Availability Organization Email URL
1 Information Only Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States