SKIP000958 : SP13.4
| Cell Line Name | SP13.4 |
|---|---|
| Original Database URL | |
| Database | |
| Species | Human |
| Type | iPS Cell |
| Original Tissue | Skin dermal fibroblast |
| Disease Status | Diseased |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | Retrovirus | |
|---|---|---|
| Transgene | SOX2, KLF4 and OCT3/4 | |
| Feeder Cell | Yes | MEFs |
| Medium | Lines of patient-specific iPS cells were maintained by mechanical dissociation of colonies and splitting 1:3 onto feeder cells in hESC medium or by limited trypsin digestion and passaging onto Matrigel-coated plates with hESC medium pre-conditioned by mouse embryonic fibroblasts. | |
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | immunocytochemistry |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | differentiation to three germ layers |
| in vivo Differentiation | Yes | Assay | teratoma formation |
Chromosomal QC
| Karyotype | Yes | Assay | |
|---|---|---|---|
| Remaining Vector Detection Test | Yes | Type | qRT-PCR |
Stem Cell Transcriptome analysis
| Stem Cell Transcriptome analysis | Yes | Assay | qRT-PCR |
|---|
No data available
| Disease Name | Parkinson disease | ICD10 | G20 |
|---|---|---|---|
| OMIM ID | 168006 | ||
| Age | 68 | ||
| Sex | Female | ||
| Race | Unknown | ||
| Genetic Analysis | Yes | ||
Mutated Genes
| # | Name | Summary |
|---|---|---|
| 1 | LRRK2 | |
| 2 | LRRK2 |
Journal
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Adriana Sanchez-Danes | Institute for Biomedicine (IBUB), University of Barcelona, |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Available | Spanish Stem Cell Bank |