SKIP000899 : 7B1
| Cell Line Name | 7B1 |
|---|---|
| Original Database URL | |
| Database | |
| Species | Human |
| Type | iPS Cell |
| Original Tissue | Skin |
| Disease Status | Normal |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | Sendai virus | |
|---|---|---|
| Transgene | Oct3/4,Sox2,Klf4,c-Myc | |
| Feeder Cell | Yes | MMC-treated MEF(ReproCELL) |
| Medium | primate ES medium (ReproCELL) supplemented with 10 ng/ml bFGF (R&D systems) | |
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | RT-PCR (*checking the paper recommended) |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | EB formation (*checking the paper recommended) |
Chromosomal QC
| Karyotype | Yes | Assay | G-banding |
|---|---|---|---|
| Remaining Vector Detection Test | Yes | Type | RT-PCR, RT-qPCR, Western Blotting, Anti-Sev-immunostaining |
No data available
| Age | 0 | ||
|---|---|---|---|
| Sex | Male | ||
| Race | Unknown | ||
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Noemi Fusaki | DNAVEC Corporation |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Information Only |