SKIP000736 : GM25256

Cell Line NameGM25256
Original Database URL
Database
SpeciesHuman
TypeiPS Cell
Original TissueSkin
Disease StatusNormal
Description
Cell Morphologyhuman ES-like
GradeResearch Grade

Method for Establishment

VectorEpisomal vector
TransgeneOCT3/4, SOX2, KLF4, LIN28, L-MYC, p53 shRNA
Feeder CellNo
MediummTeSR1
CO2(%)5%

Detection of Contaminants

Mycoplasma Negative Method PCR

Pluripotency Tests

Pluripotent MarkersYes Assay Surface antigen expression and alkaline phosphatase
in vitro DifferentiationYes Assay EB formation assay and Pluritest
in vivo DifferentiationNo Assay

Chromosomal QC

KaryotypeYes Assay G-banding

Cell Identity

STRYes Type **********

Stem Cell Transcriptome analysis

Stem Cell Transcriptome analysisYes Assay Real-time PCR

No data available
Age30
SexMale
RaceAsian : Japanese
No data available

Contact Information

# Name Organization Contact Email
1 Faith R Kreitzer Gladstone Institute of Cardiovascular Disease, UCSF

Information Provider

# Name Organization Contact Email
1 Bruce R. Conklin Gladstone Institute of Cardiovascular Disease

Provider/Distributor

# Availability Organization Email URL
1 Available Coriell Institute https://catalog.coriell.org