SKIP000636 : HiPS-RIKEN-2F

Cell Line NameHiPS-RIKEN-2F
Original Database URL
Database
Cell Line Name(2)HPS0014
SpeciesHuman
TypeiPS Cell
Original Tissueumbilical cord
Disease StatusNormal
Description
Cell Morphologyhuman ES-like
GradeResearch Grade

Method for Establishment

VectorRetrovirus
TransgenepMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
Feeder CellYesMEF (X-rays:5000R or MMC) 1-1.5x10^(6) cells/100mm dish
MediumDMEM/HamF12(2mM L-Glutamine)+20%KSR+0.1mM NEAA+0.1mM 2-Mercaptoethanol+5ng/ml human bFGF
CO2(%)3%

Detection of Contaminants

Mycoplasma Negative Method Staining(Vero),PCR

Cell Identity

STRYes Type **********

No data available
AgeFetus
SexMale
RaceAsian : Japanese
No data available

Contact Information

# Name Organization Contact Email
1 Tsuyoshi Fujioka RIKEN BioResource Center

Information Provider

# Name Organization Contact Email
1 Yukio Nakamura RIKEN BioResource Center

Provider/Distributor

# Availability Organization Email URL
1 Available RIKEN BioResource Center http://www2.brc.riken.jp/lab/cell/detail.cgi?cell_no=HPS0014&type=1