SKIP000623 : BJ hiPSC 4 factor #5
| Cell Line Name | BJ hiPSC 4 factor #5 |
|---|---|
| Original Database URL | |
| Database | |
| Species | Human |
| Type | iPS Cell |
| Original Tissue | Skin |
| Disease Status | Normal |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | Lentivirus | |
|---|---|---|
| Transgene | OCT4, SOX2, cMYC, KLF4 +GFP | |
| Feeder Cell | Yes | MEF |
| Medium | DMEM with 10% FBS, nonessential amino acids, glutamine, and beta-mercaptoethanol | |
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | Immunofluorescence analysis |
| in vivo Differentiation | Yes | Assay | teratoma formation assay |
| Other(1) | Yes | Assay | EB formation |
| Other(2) | Yes | Assay | Quantitative RT-PCR |
Chromosomal QC
| Karyotype | Yes | Assay | G-band |
|---|---|---|---|
| Remaining Vector Detection Test | Yes | Type |
No data available
| Age | 0-9 | ||
|---|---|---|---|
| Sex | Male | ||
| Race | Unknown | ||
Journal
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Nimet Maherali | Massachusetts General Hospital Center |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Information Only |