SKIP000612 : M3F-2
| Cell Line Name | M3F-2 |
|---|---|
| Original Database URL | |
| Database | |
| Species | Human |
| Type | iPS Cell |
| Original Tissue | Skin |
| Disease Status | Diseased |
| Description | |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
Method for Establishment
| Vector | Lentivirus | |
|---|---|---|
| Transgene | FUW-tetO 3 factors (OCT4, SOX2, KLF4) | |
| Feeder Cell | Yes | mitomycin C (MMC)-inactivated mouse embryonic fibroblast (MEF) feeder layers |
| Medium | hESC medium (DMEM/F12 [Invitrogen] supplemented with 15% FBS [Hyclone], 5% KnockOut Serum Replacement [Invitrogen], 1 mM glutamine [Invitrogen], 1% nonessential amino acids [Invitrogen], 0.1 mM β-mercaptoethanol [Sigma], and 4 ng/ml FGF2 [R&D Systems]) | |
Pluripotency Tests
| Pluripotent Markers | Yes | Assay | |
|---|---|---|---|
| in vitro Differentiation | Yes | Assay | |
| in vivo Differentiation | Yes | Assay | teratoma formation |
Chromosomal QC
| Karyotype | Yes | Assay | |
|---|---|---|---|
| Remaining Vector Detection Test | Yes | Type |
Stem Cell Transcriptome analysis
| Stem Cell Transcriptome analysis | Yes | Assay | Microarray Gene Expression Analysis |
|---|
No data available
| Disease Name | Dyskeratosis congenita | ICD10 | Q82.8 |
|---|---|---|---|
| OMIM ID | 305000 | ||
| Age | 30 | ||
| Sex | Female | ||
| Race | Caucasian | ||
| Genetic Analysis | Yes | ||
Cell lines with isogenic background1
Journal
Contact Information
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Rudolf Jaenisch | The Whitehead Institute |
Information Provider
| # | Name | Organization | Contact Email |
|---|---|---|---|
| 1 | Rudolf Jaenisch | The Whitehead Institute |
Provider/Distributor
| # | Availability | Organization | URL | |
|---|---|---|---|---|
| 1 | Available | The Whitehead Institute |