SKIP000309 : MJD1-9

Cell Line NameMJD1-9
Original Database URL
Database
SpeciesHuman
TypeiPS Cell
Original TissueSkin
Disease StatusDiseased
Description
Cell Morphologyhuman ES-like
GradeResearch Grade

Method for Establishment

VectorRetrovirus
TransgeneOCT4, SOX2, KLF4, c-MYC
Feeder CellYesMEF
MediumDMEM/F12 culture medium supplemented with 20% KnockOut Serum Replacement, 0.1mM non-essential amino acids (all from Invitrogen), 1mM L-glutamine, 0.1mM beta-mercaptoethanol and 50 ng/ml zebrafish basic fibroblast growth factor (zbFGF)

Pluripotency Tests

Pluripotent MarkersYes Assay ICC
in vitro DifferentiationNo Assay
in vivo DifferentiationYes Assay teratoma formation

Chromosomal QC

KaryotypeYes Assay SNP analysis
Remaining Vector Detection TestNo Type

Cell Identity

STRNo Type **********
HLANo Type **********

Stem Cell Transcriptome analysis

Stem Cell Transcriptome analysisNo Assay

No data available
Disease NameSpinocerebellar ataxia type 3 / Machado-Joseph diseaseICD10G112
OMIM ID109150
Age30-39
SexMale
EthnicityUnknown
Genetic Analysis Yes

Mutated Genes

# NameSummary
1 Ataxin 3


Contact Information

# Name Organization Contact Email
1 Oliver Brustle University of Bonn and Hertie Foundation

Provider/Distributor

# Availability Organization Email URL
1 Information Only University of Bonn and Hertie Foundation