SKIP000298 : L2-2Mut

Cell Line NameL2-2Mut
Original Database URL
Database
SpeciesHuman
TypeiPS Cell
Original TissueSkin
Disease StatusDiseased
Description
Cell Morphology--
GradeResearch Grade

Method for Establishment

VectorRetrovirus
TransgeneOSKM
Feeder CellYesMEF

Pluripotency Tests

Pluripotent MarkersYes Assay Immunostaining, qRT-PCR
in vitro DifferentiationYes Assay EB-mediated differentiation
in vivo DifferentiationYes Assay Teratoma formation
Other(1)Yes Assay differentiation into midbrain dopaminergic neuron (Immunostaining, qRT-PCR)
Other(2)Yes Assay Function of iPS cell derived neuron (Neurite outgrowth, 6-Hydroxydopamine sensitivity, Rotenone sensitivity)
Other(3)Yes Assay Expression of mRNA and protein

Chromosomal QC

KaryotypeYes Assay microarray profiling of single-nucleotide polymorphisms
Remaining Vector Detection TestYes Type qRT-PCR (silencing of the retroviral vectors)

No data available
Disease NameParkinson's diseaseICD10G20
OMIM ID607060
Age--
SexFemale
RaceUnknown
Genetic Analysis Yes

Mutated Genes

# NameSummary
1 LRRK2


Contact Information

# Name Organization Contact Email
1 Thomas Gasser Department of Neurodegenerative Diseases Hertie Institute for Clinical Brain Research, University of Tubingen, and German Center for Neurodegenerative Diseases

Provider/Distributor

# Availability Organization Email URL
1 Information Only